Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]

CAT#: AM08135FC-N

Bu-1b mouse monoclonal antibody, clone 5-11G2, FITC

Specifications

Product Data

• Clone Name: 5-11G2
• Applications: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
• Reactivities: Chicken
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.
• Specificity: This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q90747
• Background: Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.
• Synonyms: B6.2, chicken B-cell marker