Bu-1b Mouse Monoclonal Antibody [Clone ID: 5-11G2]
Bu-1b mouse monoclonal antibody, clone 5-11G2, FITC
|Recommended Dilution||Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)|
|Immunogen||Bursal cells from 2- to 5-week-old CHA (Bu-1b) strain Chickens.|
|Specificity||This antibody recognizes Chicken Bu-1b alloantigen (Mr. 70 kDa). It reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa.|
|Formulation||PBS containing 0.09% Sodium Azide as preservative.
State: Liquid purified Ig fraction.
Label: Fluorescein Isothiocyanate Isomer 1
|Storage||Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||Bu1b is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has no recognizable similarity to known mammalian molecules and therefore represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. Chicken Bu1b, a product of the Bu1b allele, is expressed on the cell surface as a disulfide linked homodimer. In 10 week old H.B15 and H.B2 chickens, Bu1 is found on 85 to 90% of bursal cells, 2 to 8% of thymocytes, 15 to 27% of spleen cells, and 2 to 18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In chickens heterozygous for the Bu1 alleles (Bu1 a/b), Bu1a does not exhibit allelic exclusion.|
|Synonyms||B6.2, chicken B-cell marker|
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