LOC396098 Mouse Monoclonal Antibody [Clone ID: 21-1A4]
Specifications
| Product Data | |
| Clone Name | 21-1A4 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1) Immunoprecipitation. (Ref.1) Immunohistochemistry on Frozen Sections. (Ref.3) |
| Reactivity | Chicken |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Bursal cells from one-day-old H.B15 (Bu-1a/b) strain chickens. |
| Specificity | This antibody recognizes Chicken Bu-1a alloantigen (Mr. 70 kDa). It does not react with cells from CHA and H.B15 strains by Immunofluorescence. |
| Buffer | 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction. |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | B6.1 |
| Database Link | |
| Background | Chicken Bu-1a, a product of the Bu-1a allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes. In Chickens heterozygous for the Bu-1 alleles (Bu-1a/b), Bu-1a does not exhibit allelic exclusion. |
| Synonyms | B-Cell Marker |
| Reference Data | |
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