H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

CAT#: AM08080PU-N

H2-D1 mouse monoclonal antibody, clone 28-14-8, Purified

Specifications

Product Data

• Clone Name: 28-14-8
• Applications: CT, FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: ≤ 1 µg/10⁶ cells. (Ref.4,7,8).
  Immunoprecipitation (Ref.2,3).
  Complement-mediated cytotoxicity (Ref.1,5,6).
  Immunohistochemistry on Frozen Sections. (Ref.4).
  Blocking (Ref.6).
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H.SW mouse splenocytes.
• Specificity: This antibody is specific to an epitope in the α3 domain of H-2D^b in the presence or absence of ß2 microglobulin. It cross reacts with the α3 domain of H-2L^d, but not K^d or D^d, and with H-2D^q and/or L^q. The antibody has been shown to block H-2L^d-specific and H-2L^d-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2L^d-positive target cells by Ly-6G2-positive NK cells.
  Customer feedback: Clone 28-14-8 does not cross-react with Human cells.
• Formulation: 1.0 ml Borate Buffered Saline, pH 8.2.
  No preservatives or amine-containing buffer salts added.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: histocompatibility 2, D region locus 1
• Database Link:
  Entrez Gene 14964 Mouse
  UniProt ID P01899
• Background: The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant ß2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8⁺ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8⁺ T cells during T cell ontogeny.
• Synonyms: H-2D(B), H2-D1