Cd24a Rat Monoclonal Antibody [Clone ID: 30-F1]

CAT#: AM08069FC-S

Cd24a rat monoclonal antibody, clone 30-F1, FITC

Product Images

Immunofluorescent Staining: BALB/c spleen cells were double-stained with Rat anti-Mouse CD24-FITC and Rat anti-Mouse CD19-PE (clone 6D5), following which small lymphocytes were gated and analyzed on a FACScan (TM) flow cytometer (BDIS, San Jose, CA). Amount Used: < / = 3 ug/10e6 cells.

Specifications

Product Data

• Clone Name: 30-F1
• Applications: FC
• Recommended Dilution: Flow Cytometry (< / = 2 µg/10e6 cells) and Cell Sorting. (Ref.3,4,7-10)
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2c
• Clonality: Monoclonal
• Specificity: This antibody recognises CD24/Heat Stable Antigen (HSA).
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  Label: FITC
  State: Liquid purified Ig fraction.
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD24a antigen
• Database Link:
  Entrez Gene 12484 Mouse
  UniProt ID P24807
• Background: CD24 (also known as heat stable antigen) is a phosphatidylinositol linked cell surface glycoprotein with a polypeptide core of 30 amino acids and high carbohydrate content. The apparent molecular mass of the two forms of CD24 purified from a mouse lymphoma cell line appear to be in the range of 40-60 and 23-30 kDa. CD24 is expressed at all stages of B cell development and on most thymocytes. The absence of expression from mature T cells is closely associated with their maturation from CD4+ CD8+ CD24+ thymocytes to either CD4+ CD8- CD24- or CD4- CD8+ CD24- T cells. CD24 is also present on mouse granulocytes, monocytes, Langerhans cells and erythrocytes. Its ligand is P selectin (CD62P). CD24 may play a role in regulation of B cell proliferation and differentiation, and it has been proposed that heat stable antigen promotes homotypic adhesion between B lymphocytes. CD24 is also a positive marker of pancreatic cancer.
• Synonyms: X62 heat stable antigen, HSA