Cd5 Rat Monoclonal Antibody [Clone ID: 4H8E6]
Specifications
| Product Data | |
| Clone Name | 4H8E6 |
|---|---|
| Application | FC, FN, IHC, IP, WB |
| Recommended Dilution | Flow Cytometry: Identification and enumeration of CD5+ cells (< / = 1 µg/10e6 cells). (Ref.15) Immunoprecipitation. (Ref.15) Immunohistochemistry on Frozen Sections. (Ref.15) T Cell Activation. (Ref.15) Western blotting. (Ref.15) |
| Reactivity | Mouse |
| Antibody Host | Rat |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Specificity | This antibody is specific to CD5/Lyt-1 (Mr. 67 kDa) |
| Buffer | PBS containing 0.09% Sodium Azide as preservative. Label: FITC State: Liquid purified Ig fraction. Label: Fluorescein Isothiocyanate Isomer 1 |
| Concentration | lot specific |
| Conjugation | FITC |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD5 antigen |
| Database Link | |
| Background | CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. (Ref.1-3) It has been identified as the major ligand of the B-cell antigen CD72. (Ref.4,5) The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in Ref.6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. (Ref.7-12) It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. (Ref.13,14) |
| Synonyms | CD5, LEU1 |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
Reviews
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Citations
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