Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]
Specifications
| Product Data | |
| Clone Name | 53-6.7 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Flow Cytometry: ≤ 2 µg/106 cells. (Ref.1,6,7,9-15). Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.2-5). Immunoprecipitation (Ref.1,6). |
| Reactivity | Mouse |
| Antibody Host | Rat |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Spleen cells or thymocyte membranes |
| Specificity | This 53-6.7 antibody recognizes the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8) |
| Buffer | PBS containing 0.09% Sodium Azide as preservative Label: Biotin State: Liquid purified Ig fraction |
| Concentration | lot specific |
| Conjugation | Biotin |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD8 antigen, alpha chain |
| Database Link | |
| Background | In the Mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells. |
| Synonyms | CD8 alpha chain, CD8A, MAL |
| Reference Data | |
| Protein Categories | ADC Targets, CD Markers, Immune system diseases, Intracellular Proteins, Membrane Proteins |
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