Nck (NCK1) Mouse Monoclonal Antibody [Clone ID: 5B7]
Specifications
| Product Data | |
| Clone Name | 5B7 |
|---|---|
| Application | ELISA, FC, IHC, WB |
| Recommended Dilution | ELISA: 1/10000. Western Blot: 1/500 - 1/2000. Flow Cytometry: 1/200 - 1/400. Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000. |
| Reactivity | Human, Monkey |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Purified recombinant fragment of Human NCK1 expressed in E. Coli. |
| Specificity | Recognizes NCK1 |
| Buffer | PBS State: Purified State: Liquid purified IgG fraction Stabilizer: 0.5% protein stabilizer Preservative: 0.05% Sodium Azide |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 47 kDa |
| Gene Name | NCK adaptor protein 1 |
| Database Link | |
| Background | The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found. |
| Synonyms | Cytoplasmic protein NCK1, NCK, NCK adaptor protein 1 |
| Reference Data | |
| Protein Categories | Intracellular Proteins |
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