NQO1 Mouse Monoclonal Antibody [Clone ID: 4D12]

CAT#: AM06702SU-N

NQO1 mouse monoclonal antibody, clone 4D12, Ascites

Product Images

Western blot analysis using NQO1 mAb against human NQO1 (AA: 134-274) recombinant protein. (Expected MW is 41.3 kDa)

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  Western blot analysis using NQO1 mouse mAb against A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and Hela (5) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded testis tissues using NQO1 mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NQO1 mouse mAb with DAB staining.

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  Flow cytometric analysis of NIH/3T3 cells using NQO1 mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 4D12
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human NQO1 expressed in E. Coli.
  
• Specificity: This antibody reacts to NQO1.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
  
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 31 kDa
• Gene Name: NAD(P)H quinone dehydrogenase 1
• Database Link:
  Entrez Gene 1728 Human
  UniProt ID P15559
• Background: This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Synonyms: DIA4, NMOR1