Glucose 6 Phosphate Dehydrogenase (G6PD) Mouse Monoclonal Antibody [Clone ID: 5E12]

CAT#: AM06694SU-N

Glucose 6 Phosphate Dehydrogenase (G6PD) mouse monoclonal antibody, clone 5E12, Ascites

Product Images

Western blot analysis using G6PD mAb against human G6PD (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)

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  Western blot analysis using G6PD mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate. Western blot analysis using G6PD mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using G6PD mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using G6PD mouse mAb with DAB staining.

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  Flow cytometric analysis of MCF-7 cells using G6PD mouse mAb (green) and negative control (red).

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Specifications

Product Data

• Clone Name: 5E12
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human G6PD expressed in E. Coli.
  
• Specificity: This antibody reacts to G6PD.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 59 kDa
• Gene Name: glucose-6-phosphate dehydrogenase
• Database Link:
  Entrez Gene 2539 Human
  UniProt ID P11413
• Background: This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Glucose-6-phosphate 1-dehydrogenase, Glucose-6-P-Dehydrogenase