p38 (CRK) Mouse Monoclonal Antibody [Clone ID: 3G11E8]

CAT#: AM06668SU-N

p38 (CRK) mouse monoclonal antibody, clone 3G11E8, Ascites

Product Images

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK antibody Cat.-No AM06668SU-N with DAB staining.

• 

  Immunohistochemical analysis of paraffin-embedded intima cancer tissues using CRK antibody Cat.-No AM06668SU-N with DAB staining.

• 

  Immunofluorescence analysis of 3T3-L1 cells using CRK antibody Cat.-No AM06668SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• 

  Flow Cytometric analysis of Hela cells using CRK antibody Cat.-No AM06668SU-N (blue) and negative control (red).

Specifications

Product Data

• Clone Name: 3G11E8
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Flow Cytometry: 1/200 - 1/400.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CRK expressed in E. Coli.
• Specificity: This antibody recognizes CRK.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 42 kDa
• Gene Name: v-crk avian sarcoma virus CT10 oncogene homolog
• Database Link:
  Entrez Gene 1398 Human
  UniProt ID P46108
• Background: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
• Synonyms: Proto-oncogene C-crk, p38