alpha smooth muscle Actin (ACTA2) Mouse Monoclonal Antibody [Clone ID: 4F4]

CAT#: AM06575SU-N

alpha smooth muscle Actin (ACTA2) mouse monoclonal antibody, clone 4F4, Ascites

Product Images

Western blot analysis using ACTA2 antibody Cat.-No AM06575SU-N against Hela (1), and Cos7 (2) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded liver tissues (left) and lung cancer tissues (right) using ACTA2 antibody Cat.-No AM06575SU-N with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and stomach tissues (right) using ACTA2 antibody Cat.-No AM06575SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 4F4
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human ACTA2 expressed in E. Coli.
• Specificity: Recognizes ACTA2
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 42 kDa
• Gene Name: actin, alpha 2, smooth muscle, aorta
• Database Link:
  Entrez Gene 59 Human
  UniProt ID P62736
• Background: The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Synonyms: ACTSA, ACTVS, Alpha-actin-2