PDK1 Mouse Monoclonal Antibody [Clone ID: 4A11]

CAT#: AM06566SU-N

PDK1 mouse monoclonal antibody, clone 4A11, Ascites

Product Images

Western blot analysis using PDK1 antibody Cat.-No AM06566SU-N against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 antibody Cat.-No AM06566SU-N with DAB staining.

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  Immunofluorescence analysis of HELA cells using PDK1 antibody Cat.-No AM06566SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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  Flow Cytometric analysis of Lovo cells using PDK1 antibody Cat.-No AM06566SU-N (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 4A11
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Flow Cytometry: 1/200 - 1/400.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human, Monkey, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human PDK1 expressed in E. Coli.
• Specificity: Recognizes PDK1
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 44 kDa
• Database Link:
  Entrez Gene 5163 Human
  UniProt ID Q15118
• Background: Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.
• Synonyms: Pyruvate dehydrogenase kinase isoform 1