Cyclin B1 (CCNB1) Mouse Monoclonal Antibody [Clone ID: 1B10]

CAT#: AM06530SU-N

Cyclin B1 (CCNB1) mouse monoclonal antibody, clone 1B10, Ascites

Product Images

Western blot analysis using Cyclin B1 antibody Cat.-No AM06530SU-N against Hela (1), Jurkat (2), K562 (3) and PC-12 (4) cell lysate.

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  Immunohistochemical analysis of paraffin-embedded lung cancer (left) and ovary tumour tissues (right) using Cyclin B1 antibody Cat.-No AM06530SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 1B10
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human CCNB1 expressed in E. Coli.
• Specificity: Recognizes Cyclin B1 (CCNB1)
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 60 kDa
• Gene Name: cyclin B1
• Database Link:
  Entrez Gene 891 Human
  UniProt ID P14635
• Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .
• Synonyms: CCNB1, CCNB, Cyclin-B1