GFAP Mouse Monoclonal Antibody [Clone ID: 6A6]

CAT#: AM06520SU-N

GFAP mouse monoclonal antibody, clone 6A6, Ascites

Product Images

Western blot analysis using GFAP antibody Cat.-No AM06520SU-N against A431 (1), SK-N-SH (2) and PC12 (3) cell lysate.

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  Immunofluorescence analysis of paraffin-embedded lobe of brain tissues using GFAP antibody Cat.-No AM06520SU-N (green).

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  Immunohistochemical analysis of paraffin-embedded brain tissues using GFAP antibody Cat.-No AM06520SU-N with DAB staining

Specifications

Product Data

• Clone Name: 6A6
• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of Human GFAP expressed in E. Coli.
• Specificity: Recognizes GFAP
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 50 kDa
• Gene Name: glial fibrillary acidic protein
• Database Link:
  Entrez Gene 2670 Human
  UniProt ID P14136
• Background: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.Tissue specificity: Expressed in cells lacking fibronectin.ABCAM:It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
• Synonyms: Glial Fibrillary Acidic Protein