HDAC3 Mouse Monoclonal Antibody [Clone ID: 7G6C5]

CAT#: AM06267SU-N

HDAC3 mouse monoclonal antibody, clone 7G6C5, Ascites

Product Images

Western blot analysis using HDAC3 antibody Cat.-No AM06267SU-N against Hela (Lane 1), NIH/3T3 (Lane 2), C6 (Lane 3) and COS (Lane 4) cell lysate.

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  Confocal Immunofluorescence analysis of Hela cells using HDAC3 antibody Cat.-No AM06267SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Specifications

Product Data

• Clone Name: 7G6C5
• Applications: IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli.
• Specificity: Recognizes HDAC3
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: histone deacetylase 3
• Database Link:
  Entrez Gene 8841 Human
  UniProt ID O15379
• Background: Histone deacetylase 3, also known as HD3, RPD3, RPD3-2. Entrez Protein NC_000005. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
• Synonyms: Histone deacetylase 3, RPD3-2, SMAP45, HD3