IKBKE Mouse Monoclonal Antibody [Clone ID: 6B4B5]

CAT#: AM06206SU-N

IKBKE mouse monoclonal antibody, clone 6B4B5, Ascites

Product Images

Western blot analysis using IKBKE antibody Cat.-No AM06206SU-N against truncated IKBKE recombinant protein (Lane 1) and full-length IKBKE (aa1-716)-hIgGFc transfected COS7 cell lysate (Lane 2).

Specifications

Product Data

• Clone Name: 6B4B5
• Applications: WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blotting: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of IKBKE (aa1-257) expressed in E. Coli.
• Specificity: Recognizes IKBKE
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• Database Link:
  Entrez Gene 9641 Human
  UniProt ID Q14164
• Background: Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon.The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degra-dation of IκB. IκB kinase α(IKKα), previously designated CHUK, interacts with IκB-αand specifically phosphorylates IκB-αon the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβand IKKγ(also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. IKK-i is a serine/threonine kinase that shares homology with IKKαand IKKβ. IKK-i is pri-marily expressed in immune cells and is induced by lipopolysaccharide and by proinflammatory cytokines including TNFα, IL-1 and IL-6. Overexpression of IKK-i was shown to result in phosphorylation of IκBαon Ser32 andSer36, and in NFκB activation, suggesting that IKK-i may act as an IκB kinase in the immune system.
• Synonyms: I kappa-B kinase epsilon, IKK-epsilon, IKK-E, IKKI, KIAA0151, Inducible I kappa-B kinase, IKK-I