ELK1 Mouse Monoclonal Antibody [Clone ID: 7E10D5]

CAT#: AM06147SU-N

ELK1 mouse monoclonal antibody, clone 7E10D5, Ascites

Product Images

Western blot analysis using ELK1 antibody Cat.-No AM06147SU-N against truncated ELK1 recombinant protein (Lane 1) and K562 cell lysate (Lane 2).

Specifications

Product Data

• Clone Name: 7E10D5
• Applications: ELISA, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of ELK1 expressed in E. Coli.
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ELK1, ETS transcription factor
• Database Link:
  Entrez Gene 2002 Human
  UniProt ID P19419
• Background: The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily,which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus,It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
• Synonyms: Elk-1