IL12B Mouse Monoclonal Antibody [Clone ID: CC326]

CAT#: AM05876AF-N

IL12B mouse monoclonal antibody, clone CC326, Azide Free

Specifications

Product Data

• Clone Name: CC326
• Applications: ELISA, FC, FN
• Recommended Dilution: ELISA: 1/100-1/1000.
  Flow Cytometry: Use 10 µl of 1/1000 diluted antibody to label 10e6 cells of 100 µl.
  Membrane permeabilisation is required.
  Functional assays: 1/100.
• Reactivities: Bovine, Human, Sheep
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Recombinant Bovine IL-12.
  Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.
• Formulation: PBS, pH 7.4
  State: Azide Free
  State: Liquid purified Ig fraction from Tissue Culture Supernatant
  Stabilizer: None
  Preservative: None
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 281857 Bovine
  UniProt ID P46282
• Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.
• Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2