Hexokinase 1 (HK1) Mouse Monoclonal Antibody [Clone ID: 4D7]

CAT#: AM05641PU-N

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Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified

 Product Datasheet for 'AM05641PU-N'

  View other "4D7" antibodies (1)

USD 510.00

5 Days

    • 100 ug


Product Data
Clone Name 4D7
Applications ELISA, IF, IHC, WB
Recommend Dilution ELISA.
Western blot (1:1,000).
Immunofluorescence (1:1,000).
Immunohistochemistry on Paraffin Sections (5 µg/ml):
This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Reactivity Human
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Recombinant Human Hexokinase 1 (1-917 aa) purified from E.coli
Specificity The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.
Formulation PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State: Purified
State: Liquid purified Ig fraction.
Concentration 1.0 mg/ml
Purification Protein-G affinity chromatography
Gene Name hexokinase 1
Background Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.
Synonyms HK1, Hexokinase type I, Brain form hexokinase
Reference Data

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