Hexokinase 1 (HK1) Mouse Monoclonal Antibody [Clone ID: 4D7]
Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified
Product Datasheet for 'AM05641PU-N'
|Applications||ELISA, IF, IHC, WB|
Western blot (1:1,000).
Immunohistochemistry on Paraffin Sections (5 µg/ml):
This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
|Immunogen||Recombinant Human Hexokinase 1 (1-917 aa) purified from E.coli|
|Specificity||The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.|
|Formulation||PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
State: Liquid purified Ig fraction.
|Purification||Protein-G affinity chromatography|
|Gene Name||hexokinase 1|
|Background||Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.|
|Synonyms||HK1, Hexokinase type I, Brain form hexokinase|
|AM05641PU-S||Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified||