SLC44A1 Mouse Monoclonal Antibody [Clone ID: VIM-15b]
SLC44A1 mouse monoclonal antibody, clone VIM-15b, Purified
|Applications||FC, IHC, IP|
|Recommended Dilution||Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 1x106 cells in 100 µl.
Immunohistochemistry on Frozen Sections.
Positive Control Tissue: Human tonsil.
|Immunogen||MV4-11 acute monocyte leukaemia cells.
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
|Specificity||This antibody is specific for the C-terminal variant of Human CDw92 (isoform 3), also known as CTL1.
Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10.
|Formulation||PBS, pH 7.4
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Preservative: 0.09% Sodium Azide
|Purification||Affinity Chromatography on Protein G|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||CDw92 is a 70kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.
CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.