SLC44A1 Mouse Monoclonal Antibody [Clone ID: VIM-15b]

CAT#: AM05591FC-N

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SLC44A1 mouse monoclonal antibody, clone VIM-15b, FITC

Conjugation: FITC PE



USD 489.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Clone Name VIM-15b
Applications FC
Recommended Dilution Flow Cytometry: Use 10 µl of neat-1/5 diluted antibody to label 1x106 cells in 100 µl.
Reactivities Human
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen MV4-11 acute monocyte leukaemia cells. 
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Specificity This antibody is specific for the C-terminal variant of Human CDw92 (isoform 3), also known as CTL1.
Clone VIM-15b is reported to augment the LPS-induced production of IL-10 by monocyte-derived dendritic cells (Mo-DCs), and the reduced expression of CDw92 by Mo-DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL-10.  
Removal of Sodium Azide is recommended prior to use in Functional Assays.
Formulation PBS, pH 7.4
Label: FITC
State: Liquid purified IgG fraction from Tissue Culture Supernatant
Stabilizer: 1% BSA
Preservative: 0.09% Sodium Azide
Label: Fluorescein Isothiocyanate Isomer 1
Absorption emission: 490 nm / 525 nm
Concentration lot specific
Purification Affinity Chromatography on Protein G
Conjugation FITC
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. 
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CDw92 is a 70kDa multi-pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells.
CDw92 is a member of the choline transporter-like protein family, so called due to their involvement in the efficient supply/transport of the natural amine choline, a vital cell nutrient required for the synthesis of cell membrane phospholipid components and the neurotransmitter acetylcholine.
Synonyms CDw92, CTL1
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.