III Mouse Monoclonal Antibody [Clone ID: E1]
Specifications
| Product Data | |
| Clone Name | E1 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | ELISA. Western blot: 1-5 µg/ml. Flow Cytometry: 1 µg/106 cells. Immunohistochemistry. |
| Reactivity | M13 Bacteriophage |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with isolated M13 phage coat proteins and mouse myeloma cells. |
| Specificity | This antibody recognizes M13 bacteriophage (gp3). |
| Buffer | Phosphate buffered saline with 0.08% Sodium Azide as preservative Label: Biotin State: Liquid purified IgG fraction |
| Concentration | lot specific |
| Purification | Protein A/G Chromatography |
| Conjugation | Biotin |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. |
| Synonyms | Attachment protein G3P, Gene 3 protein, Minor coat protein A |
| Reference Data | |
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