hnRNP K (HNRNPK) Mouse Monoclonal Antibody [Clone ID: F45 P9 C7]
$775.00
5 Days*
Specifications
| Product Data | |
| Clone Name | F45 P9 C7 |
|---|---|
| Application | IHC, WB |
| Recommended Dilution | Western Blot: 1-2 µg/ml. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Tissues: 1-5 µg/ml. Positive Controls: Colorectal cancer tissue, Calu6 and HT29 cell lysates. |
| Reactivity | Chicken, Human, Mouse, Rabbit, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the hnRNP-K protein and Mouse myeloma Ag8563 cells. |
| Specificity | This antibody reacts to Heterogeneous Nuclear Ribonucleoprotein K. |
| Buffer | PBS State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A/G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Predicted Protein Size | 51 kDa |
| Gene Name | heterogeneous nuclear ribonucleoprotein K |
| Database Link | |
| Background | Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription and premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA Polymerase II. There are approximately 20 known hnRNP proteins, which range in size from 34 kDa to 120 kDa, and their complexes are the major constituents of the spliceosome. The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling. |
| Synonyms | HNRPK, Heterogeneous nuclear ribonucleoprotein K, TUNP |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.