dam Mouse Monoclonal Antibody [Clone ID: RB1]
Specifications
| Product Data | |
| Clone Name | RB1 |
|---|---|
| Application | WB |
| Recommended Dilution | Western Blot: 5-10 µg/ml. |
| Reactivity | Bacteria |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Recombinant DNA Adenine Methylase protein. |
| Specificity | Gram Negative Bacteria. |
| Buffer | PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. |
| Synonyms | DAM |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.