FITC Mouse Monoclonal Antibody [Clone ID: F4/1]
Specifications
| Product Data | |
| Clone Name | F4/1 |
|---|---|
| Application | ELISA, FC, WB |
| Recommended Dilution | ELISA. Western Blot (1-2 µg/ml). Flow Cytometry Staining. Immunohistochemistry on Frozen and Formalin/Paraffin tissues (2-4 µg/ml). Positive Control: Antibodies labeled with FITC. |
| Reactivity | FITC |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | F4/1 is a hybridoma formed by fusing SP-2/0 Agl4 cells with spleen cells of a BALB/c mouse that had been immunised with FITC-KLH. |
| Specificity | The F4/1 antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxyfluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. When conjugated to biotin or enzyme-labeled, this antibody is also suitable for the conversation of fluorescein-labeled detection into immunoenzymatic assays. |
| Buffer | PBS containing containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Synonyms | Fluorescein Isothiocyanate |
| Reference Data | |
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