EDG6 (S1PR4) Mouse Monoclonal Antibody [Clone ID: 1]

CAT#: AM05330PU-N

EDG6 (S1PR4) mouse monoclonal antibody, clone 1, Purified

Product Images

Figure 1. Western blot analysis using EDG-6 antibody on cell lysate transfected with EDG-6 Protein at 5 ug/ml.

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  Figure 2. EDG6 antibody staining of transiently transfected CHO cells by Immunofluorescence. Developed with Cy5 anti-Mouse, nuclei are stained with DAPI.

Specifications

Product Data

• Clone Name: 1
• Applications: FC, IF, WB
• Recommended Dilution: Western Blot: 5-10 µg/ml.
  Flow Cytometry.
  Immunocytochemistry/Immunofluorescence: transiently trasfected CHO cells.
  Positive Control: RH7777 cells transfected with EDG-6 protein.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with Unique synthetic peptide derived from the C-terminal of the EDG6 (S1P4) protein and mouse myeloma cells.
• Formulation: PBS containing containing 0.08% Sodium Azide as preservative
  State: Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Upon receipt, store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: sphingosine-1-phosphate receptor 4
• Database Link:
  Entrez Gene 8698 Human
  UniProt ID O95977
• Background: EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG6 (S1P4) receptor is the sphingosine 1-phosphate receptor-4 (S1P4).
• Synonyms: EDG-6