6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: IPA2C5.1]
SKU
AM05288PU-N
6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone IPA2C5.1, Purified
$775.00
5 Days*
Specifications
| Product Data | |
| Clone Name | IPA2C5.1 |
|---|---|
| Application | WB |
| Recommended Dilution | Western Blot. |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells. |
| Specificity | This antibody is effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ. Specific for 6x-His tags only and for His tags no longer than 6 histidines. |
| Buffer | PBS containing 0.08% Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified IgG fraction. |
| Concentration | lot specific |
| Conjugation | Unconjugated |
| Storage | Store the antibody at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Background | 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.