CDC25A Mouse Monoclonal Antibody [Clone ID: DCS-121]

CAT#: AM05219PU-N

CDC25A mouse monoclonal antibody, clone DCS-121, Purified

Specifications

Product Data

• Clone Name: DCS-121
• Applications: IP, WB
• Recommended Dilution: Western Blot.
  Immunoprecipitation (1-10 ug/ml).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified recombinant CDC25A protein.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  Entrez Gene 993 Human
  UniProt ID P30304
• Background: CDC25A recognizes a 65kD protein In the cell cycle of eukaryotes the M phase is initiated by maturation promoting factor (MPF), a protein kinase complex consisting of p34 cdc2, cyclin B, and p13. The activity of this complex is controlled by multiple levels of phosphorylation and dephosphorylation of the catalytic p34cdc2 component. CDC25A and CDC25B arep34cdc2 tyrosine phosphates. Dephosphorylation of p34cdc2 by these CDC25 proteins activates the MPF complex, initiating mitosis. Although expressed throughout the cell cycle, peak expression of CDC25A is in G1 of the cell cycle.
• Synonyms: CDC-25A