Integrin alpha E (ITGAE) Mouse Monoclonal Antibody [Clone ID: AX.14]
$2,925.00
5 Days*
Specifications
| Product Data | |
| Clone Name | AX.14 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry. Monitoring of Mucosa-associated T Lymphocytes. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Specificity | This antibody reacts with Intestinal Lymphocytes (HML-1). |
| Buffer | PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative. Label: FITC State: Liquid purified IgG fraction. Label: Fluorescein Isothiocyanate Isomer 1 |
| Concentration | lot specific |
| Conjugation | FITC |
| Storage | Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | integrin subunit alpha E |
| Database Link | |
| Background | The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood ymphocytes. CD103 is expressed by 0.5 to 2% of resting lymphocytes in Peripheral blood and lymphoid organs. |
| Synonyms | Integrin alpha-E, Integrin alpha-IEL, HML-1 antigen, ITGAE |
| Note | Protocol: PBMC: Add 10 µl of MAB/10e6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2-8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20°C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.