HLA Class II DR Mouse Monoclonal Antibody [Clone ID: 423L]

SKU AM05204PU-N

HLA Class II DR mouse monoclonal antibody, clone 423L, Purified

Product Data

• Clone Name: 423L
• Application: FC
• Recommended Dilution: Flow Cytometry.; Monitoring of B cells in peripheral blood.; Analysis of B subsets.; Study of B cell activation.; Study of B cell neoplasms.
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: This antibody recats with human B and T subset Lymphocyte.
• Buffer: PBS containing 0.08% Sodium Azide as preservative.; State: Purified; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Synonyms: HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
• Note: Protocol: PBMC:; Add 10 ml of MAB/10e6 PBMC in 100 ml PBS.; Mix gently and incubate for 15 minutes at 2-8°C.; Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.; ; WHOLE BLOOD:; Add 10 ml of MAB/100 ml of whole blood.; Mix gently and incubate for 15 minutes at room temperature (20°C).; Lyse the whole blood.; Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.; See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Reference Data