OriGene Logo

HLA Class II DR Mouse Monoclonal Antibody [Clone ID: 423L]

Be the first to review this product
SKU
AM05204FC-N
HLA Class II DR mouse monoclonal antibody, clone 423L, FITC
Size:
100 Tests
Conjugation:
FITC Biotin Unconjugated
  $560.00
5 Days*
Size
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 423L
Application FC
Recommended Dilution Flow Cytometry.
Monitoring of B cells in peripheral blood.
Analysis of B subsets.
Study of B cell activation.
Study of B cell neoplasms.
Reactivity Human, Monkey
Antibody Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866.
Specificity This antibody recats with Human B and T subset Lymphocyte. Other species not tested.
Buffer PBS containing 0.2% protein carrier and 0.08% Sodium Azide as preservative.
Label: FITC
State: Liquid purified IgG fraction
Concentration lot specific
Purification Protein A/G Chromatography
Conjugation FITC
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Background Identification of human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.
Synonyms HLA-DR, HLA class II histocompatibility antigen DR, MHC class II antigen DR
Note Protocol: PBMC:
Add 10 ul of MAB/10e6 PBMC in 100 ul PBS.
Mix gently and incubate for 15 minutes at 2-8°C.
Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.

WHOLE BLOOD:
Add 10 ul of MAB/100 ul of whole blood.
Mix gently and incubate for 15 minutes at room temperature (20°C).
Lyse the whole blood.
Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze.
See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Reference Data
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Documents
Resources

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.