gamma Tubulin (TUBG1) (434-449) Mouse Monoclonal Antibody [Clone ID: TU-32]

CAT#: AM03180PU-N

gamma Tubulin (TUBG1) (434-449) mouse monoclonal antibody, clone TU-32, Aff - Purified

USD 296.00

2 Weeks*

    • 100 ug

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Product Data
Clone Name TU-32
Applications IF, WB
Recommended Dilution Western blot (1-2 µg/ml, reducing conditions).
Immunocytochemistry (Methanol/acetone fixation required).
Reactivities Mammalian, Plant, Protozoa
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human gamma-Tubulin peptide, amino acids 434-449
Specificity The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Formulation PBS, pH~7.4 containing 15 mM Sodium Azide as preservative
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% by SDS-PAGE)
Concentration lot specific
Purification Protein A Affinity Chromatography
Conjugation Unconjugated
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The γ-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing γ-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Synonyms Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.