CD16/CD32 Rat Monoclonal Antibody [Clone ID: KT1632]
CD16/CD32 rat monoclonal antibody, clone KT1632, Biotin
|Recommended Dilution||Flow Cytometry: 1 µg is the maximum amount of reagent required per 10e6 cells.
Suitable for use in single, dual or three colour Flow Cytometry.
Tissue Distribution by Flow Cytometry Analysis:
Mouse Strain: BALB/c
Cell Concentration: 1x10e6 cells per tests
Antibody Concentration Used: 0.1 µg/10e6 cells
Isotypic Control: FITC Rat IgG2a.
|Specificity||This antibody reacts with Mouse CD32/16 (Fcγ III/II receptors) which are Fc receptors present on macrophages, NK cells, monocytes, lymphocytes, and dendritic cells.
The antibody can be used to block Fc Receptor mediated binding of immunoglobulins to the FcγIII and FcγII receptors.
|Formulation||PBS containing 0.02% Sodium Azide as preservative and EIA grade BSA as a stabilizing protein.
State: Liquid purified IgG fraction.
|Storage||Store the antibody undiluted at 2-8°C for one month ore (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Background||The lymphocyte Fc-gamma Receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta.
The Fc-gamma type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression. Besides identifying Fc-gammaR+ cells, monoclonal antibodies to the Fc-gammaII/III receptor have been used to block Fc receptor binding of IgG, Fcmediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on Fc-gamma R for parasitic invasion.
|Synonyms||IgG Fc receptor III-2, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcRIIIa, Fc-gamma RIII, FcRIII, CD16a, FcR mouse Seroblock, FCGR3A, FCG3, IGFR3, Fc gamma receptor IIB, Fc-gamma-RIIB, FcRII, Ly-17, Fcgr2|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.