CD73 (NT5E) (27-252) Mouse Monoclonal Antibody [Clone ID: AT89E9]
Specifications
| Product Data | |
| Clone Name | AT89E9 |
|---|---|
| Application | ELISA, FACS, FC, ICC/IF, IF, IHC, WB |
| Recommended Dilution | ELISA. Flow Cytometry. Western blot: 1/1,000-1/2,000. Immunofluorescence/Immnunocytochemistry. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2b, kappa |
| Clonality | Monoclonal |
| Immunogen | Recombinant human CD73 (27-252aa) purified from E. coli |
| Specificity | This antibody is specific for Human NT5E/CD73. Other species not tested. |
| Buffer | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Concentration | 1mg/ml (determined by BCA assay) |
| Purification | By protein-G affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | 5'-nucleotidase ecto |
| Database Link | |
| Background | CD73 (Ecto-5-nucleotidase, 5-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells. |
| Synonyms | CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik |
| Reference Data | |
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