HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: 01G]
Specifications
| Product Data | |
| Clone Name | 01G |
|---|---|
| Application | ELISA, FC, IF, IHC, IP |
| Recommended Dilution | Flow Cytometry: 5-6 µg/ml. Immunoprecipitation. Immunohistochemistry (frozen sections). Immunocytochemistry. ELISA. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Specificity | The antibody 01G recognizes membrane-bound form of HLA-G (full-length HLA-G1), but not soluble forms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
| Buffer | PBS, pH 7.4 with 15 mM sodium azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) |
| Concentration | lot specific |
| Purification | Protein A affinity chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | major histocompatibility complex, class I, G |
| Database Link | |
| Background | HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells. |
| Synonyms | HLA-6.0, HLAG, MHC class I antigen G |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |