HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: MEM-G/2]

CAT#: AM03083PU-N

HLAG (HLA-G) mouse monoclonal antibody, clone MEM-G/2, Purified

Product Images

Western blotting analysis of HLA-G in LCL-HLA-G transfectants using anti-HLA-G (MEM-G/2).

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  Immunohistochemistry staining of kidney allograft biopsy (paraffin-embedded sections) with anti-human HLA-G (MEM-G/2).

Specifications

Product Data

• Clone Name: MEM-G/2
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry (paraffin sections).
  Western blot: Recommended dilution 1-2 μg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Bacterially expressed recombinant HLA-G heavy chain (denaturated).
• Specificity: The antibody MEM-G/2 recognizes the free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative
  State: Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, G
• Database Link:
  Entrez Gene 3135 Human
  UniProt ID P17693
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G