HLAG (HLA-G) (C-term) Mouse Monoclonal Antibody [Clone ID: 5A6G7]
$400.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 5A6G7 |
|---|---|
| Application | ELISA, FC, IF, IHC, WB |
| Recommended Dilution | Western blot (Reducing Conditions): 12% AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/ml. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISA for analysis of soluble HLA-G in combination with antibody AM03005BT-N. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin |
| Specificity | The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells. |
| Buffer | PBS, pH 7.4 with 15 mM Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE) |
| Concentration | lot specific |
| Purification | Protein A chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | major histocompatibility complex, class I, G |
| Database Link | |
| Background | HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells. |
| Synonyms | HLA-6.0, HLAG, MHC class I antigen G |
| Reference Data | |
| Atlas Protein Categories | Allergies and autoimmune diseases, Cytokines, Membrane Proteins, Secreated Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.