HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: 87G]

CAT#: AM03060FC-N

HLAG (HLA-G) mouse monoclonal antibody, clone 87G, FITC

Product Images

Surface staining of HLA-G transfectants (red) compared with K562 cells (orange) and blank (blue), with anti-HLA-G antibody (87G) biotin / streptavidin-PE.

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  Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells. Blocking of HLA-G1 on transfectants with anti-human HLA-G (87G) restored specific lysis. Target cells: M8 cell line transfected with empty vector (column 1) and with HLA-G1 cDNA (columns 2-6). Blocking antibodies: Column 1-2: none Column 3: Isotype mouse IgG2a control Column 4: anti-human HLA-G (87G) purified Column 5: anti-human HLA-G (87G) F(ab´)2 fragment Column 6: anti-human HLA-G (MEM-G/9)

Specifications

Product Data

• Clone Name: 87G
• Applications: FC
• Recommended Dilution: Flow Cytometry (1/100) analysis.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5).
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
• Concentration: lot specific
• Conjugation: FITC
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Gene Name: major histocompatibility complex, class I, G
• Database Link:
  Entrez Gene 3135 Human
  UniProt ID P17693
• Background: HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G