PRKACA Mouse Monoclonal Antibody [Clone ID: 6D2.1]
PRKACA mouse monoclonal antibody, clone 6D2.1, Aff - Purified
Product Datasheet for 'AM03041PU-N'
|Recommend Dilution||Suitable for Western Blotting (0.5-1 μg/ml).
Positive control: HeLa human cervix carcinoma cell line
Note: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
|Immunogen||Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).|
|Specificity||This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa).
The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
|Formulation||PBS, pH 7.4 with 15 mM sodium azide as preservative.
State: Aff - Purified
State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
|Purification||Protein A affinity chromatography.|
|Background||Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.|
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