PRKACA Mouse Monoclonal Antibody [Clone ID: 6D2.1]
$335.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 6D2.1 |
|---|---|
| Application | WB |
| Recommended Dilution | Suitable for Western Blotting (0.5-1 μg/ml). Positive control: HeLa human cervix carcinoma cell line Note: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis). |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha). |
| Specificity | This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog. |
| Buffer | PBS, pH 7.4 with 15 mM sodium azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). |
| Concentration | lot specific |
| Purification | Protein A affinity chromatography. |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | protein kinase cAMP-activated catalytic subunit alpha |
| Database Link | |
| Background | Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes. |
| Synonyms | PKA C-alpha |
| Reference Data | |
| Protein Categories | Cytokines, Endocrine and metabolic diseases, Enzyme: Kinases, Intracellular Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.