SHIP (INPP5D) Mouse Monoclonal Antibody [Clone ID: SHIP-01]
$335.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | SHIP-01 |
|---|---|
| Application | ELISA, WB |
| Recommended Dilution | Flow Cytometry (2-5 μg/ml) Positive control: human blood leukocytes as Western Blotting (Reducing conditions) Positive control: RAMOS human cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. ELISA. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1. |
| Specificity | The antibody SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa. |
| Buffer | PBS, pH 7.4 with 15 mM sodium azide as preservative. State: Aff - Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE). |
| Concentration | lot specific |
| Purification | Protein A affinity chromatography. |
| Conjugation | Unconjugated |
| Storage | Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | inositol polyphosphate-5-phosphatase D |
| Database Link | |
| Background | SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane. |
| Synonyms | SHIP, SHIP1, SHIP-1, p150Ship, SIP-145 |
| Reference Data | |
| Protein Families | Druggable Genome |
| Protein Pathways | B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, Phosphatidylinositol signaling system |
Reviews
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.