VCP Mouse Monoclonal Antibody [Clone ID: Hs-14]
Specifications
| Product Data | |
| Clone Name | Hs-14 |
|---|---|
| Application | FC, IF |
| Recommended Dilution | Suitable for Flow Cytometry analysis of sperm cells using 20 μl reagent / 100 μl cell suspension. The content of a vial (1 ml) is sufficient for 50 tests. |
| Reactivity | Human, Mouse |
| Antibody Host | Mouse |
| Isotype | IgM |
| Clonality | Monoclonal |
| Immunogen | Freshly ejaculated Human sperms were washed in PBS and extracted in 3% Acetic Acid, 10% Glycerol, 30mM benzaminidine. The acid extract was dialyzed against 0.2% Acetic Acid and subsequently used for immunization. |
| Specificity | The antibody reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name "intra-acrosomal protein". |
| Buffer | Tris buffered saline (TBS) containing 15 mM Sodium Azide as preservative and 0.2% (w/v) high-grade BSA (protease free) as stabilizer Label: FITC State: Liquid purified Ig fraction Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. |
| Conjugation | FITC |
| Storage | Store the antibody in the dark at 2-8°C. DO NOT FREEZE! Avoid prolonged exposure to light. |
| Stability | Shelf life: One year from despatch. |
| Shipping | Blue Ice |
| Gene Name | valosin containing protein |
| Database Link | |
| Background | VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction. |
| Synonyms | 15S Mg(2+)-ATPase p97 subunit, Valosin-containing protein |
| Reference Data | |
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