gamma Tubulin (TUBG1) Mouse Monoclonal Antibody [Clone ID: TU-30]
$365.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | TU-30 |
|---|---|
| Application | IF, WB |
| Recommended Dilution | Western blot. Immunocytochemistry (1-2 μg/ml) Staining Technique: a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Incubation Time: 45 min in room temperature. Positive Control: P-19 Mouse embryonal carcinoma cell line 3T3 mouse fibroblasts Note: The antibody TU-30 stains only fixed cells. |
| Reactivity | Bovine, Chicken, Human, Mouse, Plant, Porcine, Protozoa, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | C-terminal peptide (aa 434-449) of gamma-Tubulin counjugated to KLH |
| Specificity | The antibody TU-30 recognizes C-terminal peptide sequence of gamma-Tubulin. |
| Buffer | Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction (> 95% (by SDS-PAGE) |
| Concentration | lot specific |
| Purification | Protein-A Affinity Chromatography |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | tubulin gamma 1 |
| Database Link | |
| Background | The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The gamma-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing gamma-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface. |
| Synonyms | Tubulin gamma-1 chain, Gamma-1-tubulin, GCP-1 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.