HLA Class I ABC Mouse Monoclonal Antibody [Clone ID: W6/32]

CAT#: AM03005BT-N

HLA Class I ABC mouse monoclonal antibody, clone W6/32, Biotin

Product Images

Surface staining of human peripheral blood cells with anti-HLA-class I (W6/32) PE.

Specifications

Product Data

• Clone Name: W6/32
• Applications: ELISA, FC, ICC, IHC, IP
• Recommended Dilution: Flow Cytometry: (1/300 starting dilution).
  ELISA, IP, WB, IHC-Frozen sections, ICC: do be determined by user.
• Reactivities: Bovine, Feline, Human, Primate
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Membrane of human tonsil cells
• Specificity: The antibody W6/32 recognizes virtually all nucleated human cells. It is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.
  This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
• Formulation: PBS, pH~7.4
  Label: Biotin
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta-2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types.
• Synonyms: MHC class I ABC, HLA Class 2 ABC