Bone Sialoprotein (IBSP) (native) Mouse Monoclonal Antibody [Clone ID: ID1.2]
$195.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | ID1.2 |
|---|---|
| Application | ELISA, IF, IHC, R, WB |
| Recommended Dilution | ELISA (Cogan 2004). RIA. Western blot: 1/1,000 (Wuttke 2001, Cogan 2004, Carlinfante2003). Immunocytochemistry: 1/200 (Cogan 2004, Hilbig 2007, Graf 2012). Immunohistochemistry on Paraffin Sections: 1/400-1/2000. Immunohistochemistry on Cryo Sections, Resin-Embedded Sections: 1/100 (Ibrahim 2000, Cogan 2004, Scherberich 2007,Bauerle 2005, Mittag 2012, Carlinfante 2003, Amir 2007). |
| Reactivity | Human, Porcine, Rabbit, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Human Bone Sialoprotein (Bone extract). |
| Specificity | This antibody recognizes Human Bone Sialoprotein, Recombinant Human, Recombinant Porcine and Recombinant Rat BSP Rabbit BSP. There was no cross reactivity obtained with Human, Porcine Osteonectin and Osteopontin. |
| Buffer | 50mM TRIS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore in 10 µl aqua bidest to 1 mg/ml. |
| Purification | Protein G Chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | integrin binding sialoprotein |
| Database Link | |
| Background | The protein encoded by the Bone Sialoprotein gene is a major structural protein of the bone matrix. It is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts, and it constitutes approximately 12% of the noncollagenous proteins in human bone. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. |
| Synonyms | Bone sialoprotein II, BSP II, IBSP, BNSP |
| Note | LocusID 3381 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.