CD16 (FCGR3A) Mouse Monoclonal Antibody [Clone ID: DJ130c]

CAT#: AM01321PU-T

CD16 (FCGR3A) mouse monoclonal antibody, clone DJ130c, Purified

Product Images

Staining of Human peripheral blood lymphocytes with Mouse anti Human CD16 antibody

Specifications

Product Data

• Clone Name: DJ130c
• Applications: FC, IHC
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/20-1/200 diluted antibody to label 1x10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
  Recommended Positive Control: Human tonsil.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: CD34 antigen
• Specificity: This antibody recognizes Human CD16, a 50-65kD cell surface molecule. It recognizes all polymorphonuclear cells irrespective on their NA phenotype.
  Studies show that the DJ130c antibody recognizes an epitope in the first membrane-distal domain of CD16 and recognizes both CD16a and CD16b and has been demonstrated to cross-react with CD16 from Rhesus macaques, Macaca mulatta (Xu et al. 2012).
• Formulation: PBS
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2214 Human
  UniProt ID P08637
• Background: CD16 which is the low affinity receptor for IgG (FcRIII), exists as a transmembranous form (Fc gammaRIIIA, or CD16a) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16b). CD16a is expressed by NK cells, some T cells, and macrophages, whereas CD16b is primarily expressed by granulocytes. In addition, CD16b exists as two allelic variants NA1 and NA2.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2