CLEC10A Rat Monoclonal Antibody [Clone ID: ER-MP23]

CAT#: AM01307PU-N

CLEC10A rat monoclonal antibody, clone ER-MP23, Purified

Specifications

Product Data

• Clone Name: ER-MP23
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Flow Cytometry: Use 10 µl of 1/100-1/200 diluted antibody to label 10e6 cells in 100 µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Balb/c macrophage precursor cell hybrids.
  Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
• Specificity: This antibody recognizes murine CD301, a 38 kD cell surface protein that is otherwise known as macrophage galactose N-acetylgalactosamine lectin (MGL) or dendritic cell asialoglycoprotein (DC-ASGPR).
  Clone ER-MP23 is reported to block the function of mouse CD301. The antibody binds both MGL1 and MGL2 homologues.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-type lectin domain family 10 member A
• Database Link:
  UniProt ID Q8IUN9
• Background: In mice, CD301 is predominantly expressed on mature macrophages found associated with a wide range of connective tissues including macrophages in the dermis and the pancreas. Clone ER-MP23 also detects a population of dendritic cells in lymphoid tissue, which are probably recent immigrants from peripheral connective tissue sites. Expression of CD301 is induced by alternative (i.e. IL-4/IL-13 mediated) activation of macrophages and dendritic cells, but not all CD301 positive cells are necessarily IL-4/IL-13 stimulated.
• Synonyms: CLECSF13, CLECSF14, HML