Trypanosoma brucei Procyclin GPEET Mouse Monoclonal Antibody [Clone ID: 9G4]

CAT#: AM01249SU-N

Trypanosoma brucei Procyclin GPEET mouse monoclonal antibody, clone 9G4, Ascites

Specifications

Product Data

• Clone Name: 9G4
• Applications: ELISA, IF
• Recommended Dilution: ELISA.
  Immunofluorescence.
• Reactivities: Trypanosoma brucei
• Host: Mouse
• Isotype: IgG3
• Clonality: Monoclonal
• Immunogen: Recombinant protein containing the pentapeptidyl repeat sequence of GPEET-PARP linked to the C-terminus of glutathione-S-transferase.
• Specificity: This antibody recognizes Trypanosoma brucei procyclin GPEET.
• Formulation: State: Ascites
  State: Lyophilized Ascitic Fluid
  Stabilizer: None
  Preservative: None
• Reconstitution Method: Restore with 0.5 ml distilled sterile water.
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q95PJ0
• Background: GPEET and EP procyclins are the major surface glycoproteins of Trypanosoma brucei while it lives in the midgut (procyclic form) of tsetse flies (Glossina spp.). Trypanosoma brucei is transmitted between mammals by tsetse flies and the species causes African trypanosomiasis (or sleeping sickness). The surface of Trypanosoma brucei brucei insect forms is covered by an invariant protein coat consisting of procyclins.
  There are six or seven procyclin genes that encode unusual proteins with extensive tandem repeat units of glutamic acid (E) and proline (P) (referred to as EP repeats), and two genes that encode proteins with internal pentapeptide (GPEET) repeats. GPEET procyclin, is rich in Glu-Pro-Glu-Glu-Thr repeats. Although the EP forms of procyclins have been isolated and characterized by several laboratories, evidence for GPEET procyclin has largely been confined to the expression of its mRNA.