MICA (+ MICB) Mouse Monoclonal Antibody [Clone ID: 6D4]
$645.00
5 Days*
Specifications
| Product Data | |
| Clone Name | 6D4 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Immunoprecipitation. Functional Assays. Flow Cytometry: Use 10µl of the suggested working dilution to label 1x10e6 cells in 100µl. Immunohistochemistry on frozen sections: 1/100. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused with cells of the P3 mouse myeloma cell line. |
| Specificity | This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB). |
| Buffer | PBS, pH 7.4 State: Purified State: Liquid purified Ig |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody in aliquots at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | MHC class I polypeptide-related sequence A |
| Database Link | |
| Background | MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells. |
| Synonyms | MHC class I polypeptide-related sequence A, MIC-A, PERB11.1 |
| Note | Endotoxin Level: less than 0.01 EU/mg |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.