Bovine Corona Virus E2 protein Mouse Monoclonal Antibody [Clone ID: 5A4]

CAT#: AM00683PU-N

Bovine Corona Virus E2 protein mouse monoclonal antibody, clone 5A4, Purified


USD 734.00

2 Weeks*

Size
    • 1 mg

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Specifications

Product Data
Clone Name 5A4
Applications ELISA
Recommended Dilution ELISA.
HIT (hemagglutination inhibition titer).
Reactivities Bovine Coronavirus
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Bovine corona virus. Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Specificity Recognizes bovine corona virus surface antigen (peplomer).
Formulation PBS, pH 7.4 containing 0.09% Sodium azide
State: Purified
State: Liquid purified IgG
Concentration lot specific
Purification >95% pure (SDS-PAGE). Protein G chromatography
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Background

Bovine Corona Virus Spike glycoprotein is cleaved into S1 and S2 Spike proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R.

S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.

S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.

Synonyms Spike glycoprotein, S glycoprotein, Peplomer protein, E2 protein
Note Centrifuge before opening to ensure complete recovery of vial contents.
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.